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Mass Spectrometry Laboratory

Mass Spectrometry Laboratory (MASS Lab) is a multiuser research facility that provides access to a wide range of mass spectrometry instruments. The facility offers LC-TOF-MS, DART-TOF-MS, LC-MS-MS, GC-MS, ICP-MS and MALDI-TOF capabilities. Please contact Dr. J. S. Raaj Vellore Winfred for user training, service inquiries and sample submission.

Phone Location Hours Facility Director
(850) 644.1697 116 CSL 8:00 – 5:00 M-F Dr. J.S. Raaj Vellore Winfred

Laboratory Instruments

JEOL AccuTOF Time-of-Flight Mass Spectrometer

Description:

Time-of-flight (TOF) mass spectrometer with reflectron geometry and conventional electrospray and nano-electrospray ionization sources. Used to determine mass/charge ratios of pure compounds and components of mixtures. Equipped with ternary gradient liquid chromatograph for LC-MS analyses of complex organic, environmental, biochemical solutions.

Specifications:

  • Model: JMS-T100LC
  • Mass Range: 6 – 10,000 m/z
  • Resolution: 6000
  • Mass Accuracy: 5 ppm RMS
  • Sensitivity: S/N > 10 for 10 pg resperine

Rate:

$12/hr

Location:
CSL 116
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Waters Quattro LC-Tandem Mass Spectrometer

Description:

A high performance benchtop triple quadrupole mass spectrometer designed for routine LC-MS-MS operation for structural characterization of pure compounds or components of complex mixtures. Equipped with electrospray ionization source that can be operated in either positive or negative modes. The Quattro LC MS-MS may be coupled to a gradient HPLC system.

Specifications:

  • Model: Quattro LC
  • Inlet: Infusion pump, syringe pump, HPLC system with or without autosampler
  • Operational Modes: Daughter Ion Spectrum, Parent Ion Spectrum, Multiple Reaction Monitoring, Constant Neutral Loss Spectrum

Rate:

$12/hr

Location:
CSL 116
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Agilent 6230 TOF-MS

Description:

Agilent 6230 TOF MS provides accurate mass analyses over a wide mass range. Ideal for organic mass spectrometry. The system can be configured to work either as LC-MS or DART-MS (for rapid detection of analytes). LC-MS configuration is available only on Tuesdays.

Specifications:

  • Sensitivity, MS Mode: 1pg: 50:1 S/N reserpine
  • Mass Resolution: 22,000@1522 m/z
  • Mass Accuracy: < 1 ppm MS Mode
  • LC-MS Mass Range: m/z 25-20,000
  • DART- MS Mass Range: m/z 50-1200
  • Scan Speed: 30 scans/second

Rate:

$20/hr

Location:
CSL 1008 B
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Agilent 1260 Infinity II LC

Description:

Agilent 1260 Infinity II LC is used to separate complex chemical, biological and environmental mixtures. The system consists of a flexible pump, multisampler and variable wavelength detector. Superior quaternary mixing and a delay volume of 350 μL enable shortest run times, highest precision and accuracy.

Specifications:

  • Flow Rate: 0.001 – 5 ml/min
  • Max. Operating Pressure: 800 bar @ 5mL/min
  • Injection Range: 0.1 – 100 μL
  • Viscosity Range: 0.2 – 5 cP
  • Wavelength Detection Range: 190 – 600 nm

Rate:

$3/hr

Location:
CSL 1008 B
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Bruker Autoflex III MALDI Time-of-Flight Mass Spectrometer

Description:

Time-of-flight (TOF) mass spectrometer with matrix-assisted laser desorption/ionization (MALDI) used for identification and structural analysis of large biomolecules such as proteins, polymers, sugars, etc. The instrument has a capability of TOF/TOF technology (LIFT) for tandem MS (MS/MS), and can be utilized either linear or reflectron configuration with both positive and negative modes.

Specifications:

  • Model: AUTOFLEX III L200
  • Resolution: > 8,000
  • Mass Accuracy: < 20 ppm with extrnal calibration; < 5 ppm with internal calibration
  • Sensitivity: 500 amol [Glu1]-Fibrinopeptide (m/z 1,570) at S/N > 20

Rate:

$12/hr

Location:
CSL 1011B CSL
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Perkin Elmer Clarus 500 GC-MS

Description:

Perkin Elmer Clarus 500 GC-MS provides identification and structural characterization of volatile and semi-volatile organic compounds in complex mixtures.

Specifications:

  • Mass Range: 1 – 1200 u
  • Ionization Mode: Electron Ionization
  • Electron Ionization Voltage: 10 – 100 eV
  • Mass Stability: ± 0.1 m/z over 48 Hours
  • Scan rate: Up to 6500 amu/sec

Rate:

$12/hr

Location:
CSL 116
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Thermo Scientific iCAP RQ ICP-MS

Description:

Thermo Scientific iCAP RQ ICP-MS is capable of detecting most of the elements in the periodic table. Ideal for inorganic material analysis, geochemical exploration and pharmaceutical research.

Specifications:

  • Detection limit: < 0.5 ppt
  • Mass Range: 2 – 290 u
  • Resolution: User definable
  • Abundance Sensitivity: < 0.5 ppm @ m-1 (m= 238U)
  • Mass Stability: ≤ ± 0.025 u per day
  • Scan Speed: > 3700 amu/s; Li to U with 40 interval masses

Rate:

$20/sample

Location:
CSL 1008 B
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GE ÄKTA Pure 25 M

Description:

GE ÄKTA pure 25 M chromatography system is used for reliable purification of protiens, peptides and nucleic acids.

Specifications:

  • Flow Rate: 0.001 – 25 ml/min
  • Max. Operating Pressure: 20 MPa
  • UV Monitor: 280 nm
  • Viscosity Range: 0.35 – 10 cP

Rate:

$2.50/hr

Location:
CSL 4501 (Cold Room)
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Sorvall Centrifuge

Description:

High-speed (up to 22,000 rpm) centrifuge with fixed angle and swinging bucket rotors.

Specifications:

  • Model: RC 6 PLUS
  • Max. Speed: 22,000 rpm

Location:
CSL 116